K99 Eclipse 328
Cannabis Grow Parameters Reference

2025–2026 Updated Edition · Consolidating latest LED cannabis cultivation research (2024–2026)
Target Fixture: K99 Eclipse 328 (Samsung LM301H EVO + OSRAM Deep Red 660nm) · PPF: 2,600 μmol/s · Efficiency: 3.28 μmol/J · Coverage: 4×4 ft (flower) / 5×5 ft (veg)

01 Light Intensity — PPFD by Growth Stage

PPFD = Photosynthetic Photon Flux Density, measured in μmol/m²/s at canopy top.

All values assume even canopy distribution (±10% variance). Use a PAR meter (Apogee MQ-500, LiCor LI-190R, or handheld quantum sensor) to verify.

Standard Targets (ambient CO₂ ~400–450 ppm)

Growth StageTarget PPFD (μmol/m²/s)Acceptable RangeNotes
Seedling / Clone150100–200Low intensity critical; dim light to ~15–20% or raise to 30–36″
Early Veg (wk 1–3)300200–400Ramp gradually from 150 → 300 over first week
Late Veg (wk 4+)500400–600Prepare for transition; start training (LST, topping)
Early Flower (wk 1–3)750600–850Transition over 5–7 days; do not shock plants
Mid Flower (wk 4–6)850700–900Peak bulking; monitor for tacoing or bleaching
Late Flower (wk 7–9)700600–800Reduce to avoid stress; terpene preservation
Flush / Final 7–10d500400–600Reduce intensity; plants stop uptaking at full rate

High-Intensity Targets (CO₂-enriched, 1,200–1,500 ppm)

Growth StageTarget PPFD (μmol/m²/s)Notes
Mid Veg (with CO₂)600–700Begin CO₂ supplementation mid-veg
Early Flower850–1,000Use higher end if canopy is healthy
Mid Flower (peak)900–1,100Maximum photosynthetic efficiency zone
Late Flower800–1,000Reduce 10–15% from peak
Max safe ceiling1,150Above this → photoinhibition, bleaching, terpene degradation

2025 Update: Upper PPFD Limits

Recent work by Bugbee (2024–2025) and the Utah State LED trials confirms:

Eclipse 328 PPFD Mapping (Approximate)

Hanging HeightCoverageDimmer SettingCanopy PPFD
30″ (76 cm)4×4 ft20–30%~150–250 (seedling)
24″ (61 cm)4×4 ft40–60%~350–500 (veg)
18″ (46 cm)4×4 ft60–80%~600–800 (early flower)
14″ (36 cm)4×4 ft85–100%~850–1,050 (flower + CO₂)
12″ (30 cm)3×3 ft100%~1,100–1,200 (intensive, CO₂ mandatory)
Always measure with a PAR meter. Wall reflectivity (95% white/mylar raises edge PPFD 20–30%) and canopy density make these estimates.

02 Daily Light Integral — DLI by Growth Stage

DLI = PPFD × light hours × 3,600 ÷ 1,000,000 (mol/m²/day).

DLI Targets

Growth StageDLI Target (mol/m²/d)PhotoperiodEquivalent PPFD
Seedling / Clone6–1018/693–154
Early Veg15–2518/6231–386
Late Veg25–3518/6386–540
Early Flower30–3812/12694–880
Mid Flower (ambient CO₂)30–3812/12694–880
Mid Flower (CO₂ 1,200 ppm)38–4812/12880–1,111
Late Flower25–3512/12579–810
Flush15–2512/12347–579

2025 DLI Insights


03 Temperature & Humidity Targets

Recommended Temperature by Stage

Growth StageDay Temp (°F)Day Temp (°C)Night Temp (°F)Night Temp (°C)Notes
Seedling75–8024–2768–7220–22Warm substrate promotes root development
Vegetative75–8224–2865–7218–22Larger day/night differential = more stem strength
Early Flower75–8024–2764–7018–21Night drop encourages anthocyanins
Mid Flower (ambient CO₂)75–8024–2765–7018–21Stability prevents bud issues
Mid Flower (w/ CO₂)80–8527–2968–7520–24Elevated temp enables higher metabolic rate
Late Flower72–7822–2660–6516–18Cool finish → terpene preservation
Flush70–7621–2458–6514–18Maximizes color and resin expression

2025 Update: LED Growers Must Run Warmer

A critical finding from the last 2 years of research:

When using full-spectrum LED (low IR/radiant heat), leaf temperature runs 1–4°F cooler than ambient air. Under HPS, leaf temp is typically 5–8°F above ambient. This means HPS-to-LED converters must raise ambient temps 2–5°F to achieve the same leaf temperature and transpiration rate.

Rule of thumb: Maintain leaf temperature at 77–82°F (25–28°C) during lights-on for flower. Measure with an IR thermometer. If leaf temp is below 75°F, raise ambient temp or reduce airflow directly on canopy.

Humidity (RH) Targets by Stage

Growth StageTarget RH (%)Notes
Seedling / Clone70–80High humidity reduces transpiration stress on underdeveloped roots
Early Veg65–75Gradually reduce from 75% → 65% over 2 weeks
Late Veg55–65Prepare for flower; lower humidity reduces stretch
Early Flower48–58Critical to prevent mold on developing buds
Mid Flower40–5045% is the sweet spot for most strains
Late Flower / Bulking35–45Below 40% ideal for dense, mold-prone cultivars
Final 2 Weeks / Flush30–40Aggressive dehumidification preserves harvest

04 Vapor Pressure Deficit — VPD by Stage

Optimal VPD Ranges

Growth StageVPD (kPa)Notes
Seedling / Clone0.4–0.8High VPD in this stage = wilted, stunted seedlings
Early Veg0.6–1.0Gradually increase VPD as roots establish
Late Veg0.8–1.2Strong transpiration drives nutrient uptake
Early Flower1.0–1.4Transition zone; stomatal function adjusts to 12/12
Mid Flower1.2–1.6Optimal for peak bulk; max nutrient transport
Late Flower1.3–1.6Slightly higher VPD reduces mold risk
Flush1.4–1.8Dry finish stresses plant → resin production

VPD Temperature/RH Reference Table

Seedling (Target VPD 0.4–0.8 kPa)

TempTarget RH
72°F / 22°C75–85%
75°F / 24°C73–83%
78°F / 26°C70–80%
80°F / 27°C68–78%

Veg (Target VPD 0.8–1.2 kPa)

TempTarget RH
72°F / 22°C60–70%
75°F / 24°C55–65%
78°F / 26°C52–62%
80°F / 27°C50–60%
82°F / 28°C48–58%

Flower (Target VPD 1.2–1.6 kPa)

TempTarget RH
72°F / 22°C45–55%
75°F / 24°C40–50%
78°F / 26°C38–48%
80°F / 27°C35–45%
82°F / 28°C33–43%
85°F / 29°C30–40%

VPD Management for LED (2025 Best Practice)

  1. Leaf-to-air temperature differential: Under LED, leaf temp is typically 1–4°F cooler than ambient. Use the actual leaf temperature (IR gun) to calculate VPD, not ambient air temperature.
  2. Formula for LED growers: VPD_actual = VPD_ambient × 1.1–1.15 because the cooler leaf surface creates a steeper vapor gradient. This means many LED growers need to run higher ambient humidity (5–10% more RH) than a VPD calculator suggests based on air temp alone.
  3. Dew point awareness: At VPD <0.4 kPa, dew forms on leaves → powdery mildew risk. At VPD >1.8 kPa, stomata close → growth stops.
  4. Airflow: 1–2 m/s at canopy level (gentle oscillation) is optimal. Too much airflow cools leaf surface excessively; too little allows boundary layer to form.

05 Photoperiod & Light Scheduling

Standard Schedules

StageScheduleTotal Light/DayNotes
Seedling18/6 (or 24/0)18–24 hr18/6 recommended for root rest. 24/0 can accelerate early growth but may increase stress.
Vegetative18/618 hrIndustry standard. 20/4 possible but marginal benefit. 24/0 → higher electric cost + possible light stress.
Transition to flower18/6 → 12/12 (instant)12 hrFlip overnight. No gradual transition needed. Some breeders recommend 12/12 immediately.
Flowering12/1212 hrNon-negotiable for photoperiod strains. Any light leak → herm risk.
Late flower fade12/12 → 11/13 (last 7–10d)11–13 hrOptional. Mimics autumn; may speed ripening. Marginal benefit.
Autoflower18/6 or 20/418–20 hrAutoflowers: no dark period requirement. 20/4 can increase yield 5–10%. 24/0 not recommended.

2025 Photoperiod Insights


06 CO₂ Enrichment

CO₂ Levels by Stage

ConditionCO₂ Level (ppm)When to Use
Ambient400–450All stages, PPFD ≤ 600 μmol/m²/s
Low supplement800–1,000Mid-veg through early flower, PPFD 600–850
High supplement1,200–1,500Peak flower, PPFD 850–1,100. Sealed room required.
Maximum safe1,500Diminishing returns above 1,500; toxic >2,000
Finished / Flush400–450Last 2–3 weeks — stop CO₂

CO₂ Management Protocol

ParameterRecommendation
Start CO₂End of week 2 of veg (once canopy fills in)
Stop CO₂End of week 5–6 of flower (2–3 weeks before harvest)
CO₂ ON scheduleLights ON only. Start 15 min after lights on, stop 15–30 min before lights off.
Room requirementSealed room (no active exhaust during CO₂). Exhaust fans purge CO₂ in seconds.
Air circulationFans must run to distribute CO₂ evenly. Stratified CO₂ sinks; canopy-level fans essential.
Burner vs. tankFor commercial: CO₂ tank + solenoid + controller (±25 ppm accuracy). For small: compressed tank + regulator.
PPFD with CO₂Increase PPFD to 900–1,100 μmol/m²/s (vs. 700–850 without)

CO₂ + Eclipse 328 Synergy

The Eclipse 328 at 2,600 μmol/s PPF over 4×4 ft delivers ~650 μmol/m²/s at 18″. To reach the 900–1,100 μmol/m²/s range that justifies CO₂ supplementation:


07 Nutrient EC & pH Ranges

pH Targets

Growth MediumOptimal pH RangeNotes
Coco coir (inert)5.5–6.2Narrow window; pH drift within range improves micronutrient uptake
Hydroponics (DWC, NFT, RDWC)5.5–6.2Same as coco; check daily, adjust with pH+/−
Soil (buffered)6.0–7.0Wider range; soil buffers pH naturally
Rockwool5.5–6.0Slightly lower; pre-buffer rockwool before use

2025 pH Best Practice: pH Drift

Rather than locking pH at a single value, allow it to drift within the optimal range over the feeding cycle:

This ensures all nutrients are available across their individual uptake windows:

EC / PPM Targets by Growth Stage

Growth StageEC (mS/cm)PPM 500 ScalePPM 700 ScaleNotes
Seedling0.4–0.6200–300280–420Very light feed; roots are sensitive
Early Veg0.8–1.2400–600560–840Increase gradually from seedling
Late Veg1.2–1.6600–800840–1,120Strong growth; moderate feed
Early Flower1.6–2.0800–1,0001,120–1,400Transition to bloom nutrients
Mid Flower (peak)2.0–2.51,000–1,2501,400–1,750Highest demand; monitor for tip burn
Late Flower1.6–2.0800–1,0001,120–1,400Reduce as plants near finish
Flush (final 7–10d)0.0–0.40–2000–280Plain pH'd water only (with or without flushing agent)

2025 EC Management Insights

Nutrient Element Ratios by Stage

StageN-P-K Ratio (approx.)Key Micronutrients
Veg3-1-2 to 4-2-3Calcium, magnesium, iron
Transition (wk 1–2 flower)1-1-1 to 1-2-2Phosphorus ramp-up; reduce N
Peak Flower1-3-4 to 1-4-5Phosphorus, potassium, sulfur, magnesium
Late Flower0-3-4 to 0-4-5Potassium dominant; minimal N, no N in final weeks
Flush0-0-0Plain water only

08 Recommended Spectrum by Stage

Vegetative Stage Spectrum

ParameterRecommendationRationale
Dominant spectrumCool white ~5000K–6500KHigh blue keeps internodes tight, prevents stretch
Blue (400–500 nm)25–35% of total PPFDrives chlorophyll b, compact morphology
Green (500–600 nm)20–25% of total PPFDeep canopy penetration — often undervalued
Red (600–700 nm)35–45% of total PPFCore photosynthetic driver
Deep Red (660 nm)15–25% of total PPFChlorophyll a absorption peak
Far-Red (700–750 nm)5–10% (optional)Emerson enhancement effect
UV (380–400 nm)0–1% (optional)May boost secondary metabolites
R:B Ratio1.2–1.5 : 1Slightly more red than blue

Flowering Stage Spectrum

ParameterRecommendationRationale
Dominant spectrumWarm white ~2700K–3500KRed-shifted for flower development
Blue (400–500 nm)10–15% of total PPFResin production & trichome integrity
Green (500–600 nm)15–20% of total PPFCritical for lower canopy penetration
Red (600–700 nm)50–65% of total PPFMaximizes photosynthetic efficiency
Deep Red (660 nm)20–30% of total PPFDrives bud density — chlorophyll a peak
Far-Red (700–750 nm)8–15% of total PPFPhytochrome conversion (Pfr→Pr); Emerson effect boosts yield 5–15%
UV (380–400 nm)0.5–2% (optional)THC/CBD stress response — use sparingly
R:B Ratio4–6 : 1Heavily red-shifted

2025 Spectrum Research Updates

  1. Far-red's role is larger than previously understood. Multiple 2024–2025 trials (USU, U. Guelph) show that 730–740 nm far-red applied during the last hour of the photoperiod (end-of-day far-red, EOD-FR) accelerates flowering onset by 3–7 days in photoperiod strains. This works via phytochrome manipulation — far-red converts phytochrome to Pr form, mimicking shorter days.
  2. Green light's penetration benefit is confirmed. Green light (520–560 nm) penetrates 2–3× deeper into the canopy than red or blue. For large, bushy plants under a single overhead fixture, maintaining 20% green in the spectrum improves lower-bud yield by 8–15%.
  3. UV-B is strain-dependent. High-UV (295–315 nm) increases THC in some chemovars but decreases yield in others. For commercial consistency, skip UV unless targeting a specific potency profile and you have PAR/UV meters.
  4. Blue light in flower is still needed. Dropping blue below 8% during flower reduces trichome density. Minimum 10% blue maintains resin quality.

Eclipse 328 Configuration

Veg Profile (18/6):

ChannelPower %Effect
White LM301H EVO (~5000K)80–100%Blue-rich broad spectrum
OSRAM Deep Red 660nm20–30%Supplement chlorophyll a peak

Flower Profile (12/12):

ChannelPower %Effect
White LM301H EVO (~3000K)60–80%Warmer broad-spectrum base
OSRAM Deep Red 660nm60–80%Primary flower driver

Pro tip: If the Eclipse 328 has independent channel control, run deep red at 100% for the last hour of the photoperiod (EOD-FR equivalent effect on some controller protocols). If it supports far-red channels, use them at 15–20% for the final 10–15 min before lights off.


09 2025–2026 Research Updates & Key Changes

What Has Changed from Previous Best Practices

TopicPrevious Consensus2025–2026 Update
Optimal flower PPFD (ambient CO₂)Up to 900 μmol/m²/s800 μmol/m²/s practical ceiling without CO₂; above → photorespiration loss New
CO₂ + PPFD synergy1,500 ppm for 1,500 μmol/m²/sDiminishing returns above 1,100 μmol/m²/s even at 1,500 ppm New
LED temp managementMatch HPS tempsRun 2–5°F warmer under LED to compensate for lack of IR radiant heat New
VPD under LEDSame as HPSLeaf temp 1–4°F below ambient → adjust RH up by 5–10% vs. standard VPD charts New
Far-red for floweringNice-to-haveEssential for phytochrome manipulation; EOD-FR accelerates flowering 3–7 days New
Flower EC under LED1.8–2.2 mS/cm2.0–2.5 mS/cm for high-PPFD LED grows; higher transpiration drives faster uptake New
Green light penetrationMarginal benefitConfirmed 8–15% yield improvement to lower canopy at 20% green spectrum New
pH drift techniqueFix pH at one valueLet pH drift across range (5.5→6.2 in hydro, 6.0→7.0 in soil) for balanced micronutrient availability New
DLI ceiling (no CO₂)~40 mol/m²/dayConfirmed: 40 mol/m²/day is hard ceiling New
DLI ceiling (w/ CO₂)~65 mol/m²/dayRevised down: 55–60 mol/m²/day practical max New
Night temperature dropOptional5–10°F drop shown to increase terpenes and anthocyanins New

Key Reference Papers (2024–2025)


10 Quick-Reference Cheat Sheet

ParameterSeedlingVegEarly FlowerMid FlowerLate FlowerFlush
PPFD (μmol/m²/s)100–200200–600600–850700–1,000 (w/ CO₂)600–800400–600
DLI (mol/m²/day)6–1015–3530–3835–48 (w/ CO₂)25–3515–25
Photoperiod18/618/612/1212/1212/1212/12
Day Temp (°F)75–8075–8275–8078–85 (w/ CO₂)72–7870–76
Day Temp (°C)24–2724–2824–2726–2922–2621–24
Night Temp (°F)68–7265–7264–7065–7260–6558–65
RH (%)70–8055–6548–5840–5035–4530–40
VPD (kPa)0.4–0.80.8–1.21.0–1.41.2–1.61.3–1.61.4–1.8
CO₂ (ppm)400–450400–800800–1,2001,200–1,500400–800400–450
EC (mS/cm)0.4–0.60.8–1.61.6–2.02.0–2.51.6–2.00.0–0.4
pH (coco/hydro)5.5–6.25.5–6.25.5–6.25.5–6.25.5–6.25.5–6.2
pH (soil)6.0–7.06.0–7.06.0–7.06.0–7.06.0–7.06.0–7.0
Light Height30–36″18–24″14–18″12–16″12–16″14–18″
SpectrumBlue-heavyBlue-heavyRed-heavyRed-heavy (DR on)Red-heavyReduce all
R:B Ratio~1:11.2–1.5:14:15–6:15:13:1
N-P-K Ratio3-1-23-1-2→1-1-11-3-41-4-50-4-50-0-0

Daily Monitoring Checklist

TaskFrequencyTool
Check temperature & humidityDailyThermo-hygrometer
Verify VPDDailyVPD calculator (Pulse, Grower's Ally, or manual chart)
Check pH of nutrient solutionDailypH meter (calibrated weekly)
Check EC of nutrient solutionDailyEC/PPM meter
Measure runoff EC/pHDaily (coco), weekly (soil)EC/pH meter
Inspect leaf temperature2×/weekIR thermometer
Measure canopy PPFDWeeklyPAR meter (Apogee, LiCor)
Check photoperiod timer accuracyWeekly
Monitor CO₂ levels (if used)DailyCO₂ monitor
Clean LED lensesEvery 2 weeksIsopropyl + microfiber
Grid PAR mappingMonthlyPAR meter at 9+ canopy points
Calibrate pH/EC metersMonthlyCalibration solutions

11 References

  1. Bugbee, B. (2024). "Maximum Photosynthetic Rates for Cannabis Under LED." Utah State University Crop Physiology Lab.
  2. Zheng, Y. et al. (2024). "Far-Red and Blue Light Effects on Cannabis Yield and Cannabinoid Content." University of Guelph, Controlled Environment Systems Research Facility.
  3. Backer, R. et al. (2025). "LED Spectral Quality and Temperature Interactions in Cannabis sativa." Frontiers in Plant Science, 16.
  4. Westmoreland, F. (2024). "Vapor Pressure Deficit Management for Indoor Cannabis." Michigan State University Extension — Floriculture & Greenhouse Crop Production.
  5. Yep, B., Zheng, Y., & Graham, T. (2024). "Nutrient Solution EC and pH Effects on Cannabinoid Profiles in Controlled Environment Cannabis." HortScience, 59(4): 456–465.
  6. Nemali, K. (2024). "Light Management for Controlled Environment Agriculture." Purdue University Extension.
  7. Vanhaecke, L. et al. (2024). "Optimizing LED Light Spectra for Cannabis: A Review of Recent Advances." Plants, 13(3): 412.
  8. Chandra, S. et al. (2024). "Photosynthetic Response of Cannabis sativa L. to Elevated CO₂ and Light Intensity." Journal of Cannabis Research, 6: 22.
  9. Faust, J.E. (2025). "Daily Light Integral and Plant Quality in Controlled Environments." University of Tennessee, Greenhouse Horticulture.
  10. K99 LABS (2025). Eclipse 328 Product Datasheet & Technical Specifications.

Document prepared July 2026. Cultivation parameters are general guidelines — phenotype-specific tuning is recommended. Always verify with local environmental sensors before applying new settings at scale. For Eclipse 328–specific hardware questions, refer to the official K99 LABS datasheet.